Early keratinocyte differentiation on micropillar interfaces

Steinberg, Thorsten; Schulz, Simon; Spatz, Joachim P.; Grabe, Niels; Mussig, Eva; Kohl, Annette; Komposch, Gerda; Tomakidi, Pascal

doi:10.1021/nl062271z

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Early keratinocyte differentiation on micropillar interfaces

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Schulz, Simon
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Spatz, Joachim P.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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http://pubs.acs.org/doi/pdf/10.1021/nl062271z
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https://dx.doi.org/10.1021/nl062271z
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http://pubs.acs.org/doi/suppl/10.1021/nl062271z
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Citation

Steinberg, T., Schulz, S., Spatz, J. P., Grabe, N., Mussig, E., Kohl, A., et al. (2007). Early keratinocyte differentiation on micropillar interfaces. Nano Letters, 7(2), 287-294. doi:10.1021/nl062271z.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-432C-9

Abstract

We employed topographical patterning to analyze early keratinocyte differentiation on top of microfabricated pillar arrays. Fibronectin immobilized on pillar "heads" yielded a nucleus-associated granular keratin 1 (K1) pattern in immortalized human gingival keratinocytes (IHGK) at pillar interspaces of 14 mum. Decreasing distances of 11and 8 mum revealed cytoplasmic extension of the early differentiation marker K1 on poly(dimethylsiloxane) (PDMS) pillars. The most extensive cytoplasmic K1 protein distribution noted at the smallest pillar scale coincided with higher ratios of K1 mRNA gene transcription. These experiments suggest that early keratinocyte differentiation was governed by the topographical characteristics of the pillar pattern. Moreover, they form the basis to study cell functions such as differentiation in a defined topologically structured environment.