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The novel fluorescent CDP-analogue (Pβ)MABA-CDP is a specific probe for the NMP binding site of UMP/CMP-kinase

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Reinstein,  Jochen
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Rudolph, M. G., Veit, T., & Reinstein, J. (1999). The novel fluorescent CDP-analogue (Pβ)MABA-CDP is a specific probe for the NMP binding site of UMP/CMP-kinase. Protein Science, 8(12), 2697-2704. doi:10.1110/ps.8.12.2697.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002C-21AF-3
Abstract
Direct thermodynamic and kinetic investigations of the binding of nucleotides to the nucleoside monophosphate (NMP) site of NMP kinases have not been possible so far because a spectroscopic probe was not available. By coupling a fluorescent N-methylanthraniloyl- (mant) group to the β-phosphate of CDP via a butyl linker, a CDP analogue [(Pβ)MABA-CDP] was obtained that still binds specifically to the NMP site of UmpKdicty, because the base and the ribose moieties, which are involved in specific interactions, are not modified. This allows the direct determination of binding constants for its substrates in competition experiments.