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Journal Article

Developmentally regulated epitopes on a neurofilament protein visualized by monoclonal antibodies.

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Thorey,  I.
Abteilung Neurobiologie, MPI for biophysical chemistry, Max Planck Society;

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Seifert,  W.
Abteilung Neurobiologie, MPI for biophysical chemistry, Max Planck Society;

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Citation

Thorey, I., & Seifert, W. (1989). Developmentally regulated epitopes on a neurofilament protein visualized by monoclonal antibodies. Developmental Brain Research, 49(2), 229-241. doi:10.1016/0165-3806(89)90024-2.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002C-2AEC-8
Abstract
Two monoclonal antibodies (mabs) which recognized the 68 kDa subunit of the rat neurofilament triplet were isolated. In immunoblots with SDS-solubilized and reduced proteins, these mabs recognized their epitopes equally well in embryonic and in adult tissue. However, these epitopes were developmentally regulated in paraformaldehyde-fixed rat brain sections. They were abundant in all compartments of differentiating neurons, whereas in mature neurons their presence was markedly attenuated, with a moderate abundance in perikarya and larger dendrites and low concentrations in axons. Thus, a differential developmental modification, possibly involving the masking of an epitope, is demonstrated for the small neurofilament polypeptide in rat and monkey brain tissue.