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Journal Article

Different cAMP sources are critically involved in G protein-coupled receptor CRHR1 signaling

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Maccarrone,  Giuseppina
Dept. Translational Research in Psychiatry, Max Planck Institute of Psychiatry, Max Planck Society;

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Turck,  Christoph W.
Dept. Translational Research in Psychiatry, Max Planck Institute of Psychiatry, Max Planck Society;

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Citation

Inda, C., dos Santos Claro, P. A., Bonfiglio, J. J., Senin, S. A., Maccarrone, G., Turck, C. W., et al. (2016). Different cAMP sources are critically involved in G protein-coupled receptor CRHR1 signaling. JOURNAL OF CELL BIOLOGY, 214(2), 181-195. doi:10.1083/jcb.201512075.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002C-5BDE-A
Abstract
Corticotropin-releasing hormone receptor 1 (CRHR1) activates G protein-dependent and internalization-dependent signaling mechanisms. Here, we report that the cyclic AMP (cAMP) response of CRHR1 in physiologically relevant scenarios engages separate cAMP sources, involving the atypical soluble adenylyl cyclase (sAC) in addition to transmembrane adenylyl cyclases (tmACs). cAMP produced by tmACs and sAC is required for the acute phase of extracellular signal regulated kinase 1/2 activation triggered by CRH-stimulated CRHR1, but only sAC activity is essential for the sustained internalization-dependent phase. Thus, different cAMP sources are involved in different signaling mechanisms. Examination of the cAMP response revealed that CRH-activated CRHR1 generates cAMP after endocytosis. Characterizing CRHR1 signaling uncovered a specific link between CRH-activated CRHR1, sAC, and endosome-based signaling. We provide evidence of sAC being involved in an endocytosis-dependent cAMP response, strengthening the emerging model of GPCR signaling in which the cAMP response does not occur exclusively at the plasma membrane and introducing the notion of sAC as an alternative source of cAMP.