English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

γ-Aminobutyric acid elevates cytosolic Ca in bovine chromaffin cells.

MPS-Authors
/persons/resource/persons15004

Doroshenko,  P.A.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

Locator
There are no locators available
Fulltext (public)

2378146.pdf
(Publisher version), 294KB

Supplementary Material (public)
There is no public supplementary material available
Citation

Doroshenko, P. (1989). γ-Aminobutyric acid elevates cytosolic Ca in bovine chromaffin cells. Neuroscience Letters, 104(1-2), 83-87. doi:10.1016/0304-3940(89)90333-9.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002C-2E68-C
Abstract
Measurements of the cytosolic Ca concentration ([Ca]i) with the Ca-sensitive dye, fura-2, showed that in intact, but not in voltage-clamped, bovine chromaffin cells γ-aminobutyric acid (GABA, 10 μM) elicited a transient increase in [Ca]i. The Ca transient of intact cells was inhibited by bicuculline (20 μM), by removal of extracellular Ca or by treatment with the Ca channel blocker cobalt (2.5 mM), and enhanced by lowering the extracellular Cl. We conclude, that GABA elevates [Ca]i by inducing a GABAA-receptor-linked Cl current which depolarizes the cell membrane sufficiently to activate potential-operated Ca channels and cause Ca entry into the cell.