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Exploring productive sequence space in directed evolution using binary patterning versus conventional mutagenesis strategies.

MPG-Autoren
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Sun,  Zhoutong
Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society;

Torres Salas,  Pamela
Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society;

Siirola,  Elina
Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society;

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Lonsdale,  Richard
Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society;

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Zitation

Sun, Z., Torres Salas, P., Siirola, E., Lonsdale, R., & Reetz, M. T. (2016). Exploring productive sequence space in directed evolution using binary patterning versus conventional mutagenesis strategies. Bioresources and Bioprocessing, 3(44), 1-8. doi:10.1186/s40643-016-0122-8.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-002C-43BF-6
Zusammenfassung
Background: Recent methodology development in directed evolution of stereoselective enzymes has shown that various mutagenesis strategies based on saturation mutagenesis at sites lining the binding pocket enable the generation of small and smart mutant libraries requiring minimal screening. Methods: In this endeavor, limonene epoxide hydrolase (LEH) has served as an experimental platform, the hydrolytic desymmetrization of cyclohexene oxide being the model reaction with formation of (R,R)- and (S,S)-cyclohexane- 1,2-diol. This system has now been employed for testing reduced amino acid alphabets based on the Hecht concept of binary patterning, with and without additional hydrophobic amino acids. Results and Conclusions: It turns out that in binary pattern based saturation mutagenesis as applied to LEH, polar amino acids are seldom introduced. When applying binary patterning in combination with additional hydrophobic amino acids as building blocks in iterative saturation mutagenesis, excellent LEH variants were evolved for the production of both (R,R)- and (S,S)-diols (80–97 % ee), but again the introduction of polar amino acids occurs rarely. Docking computations explain the source of enhanced and inverted stereoselectivity. Some of the best variants are also excellent catalysts in the hydrolytic desymmetrization of other meso-epoxides, although both enantiomeric diols are not always accessible.