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Calcium content of the erythrocytes: a sensitive and easy handling method for measuring free calcium ions, and modulation of the Ca2+ ion concentration by the calcium antagonists nifedipine and pentoxifylline.

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Winkler,  A. H.
Abteilung Biochemische Kinetik, MPI for biophysical chemistry, Max Planck Society;

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Citation

Friederichs, E., Radisch, T., & Winkler, A. H. (1989). Calcium content of the erythrocytes: a sensitive and easy handling method for measuring free calcium ions, and modulation of the Ca2+ ion concentration by the calcium antagonists nifedipine and pentoxifylline. Clinical and Experimental Pharmacology and Physiology, 16(5), 387-394. doi:10.1111/j.1440-1681.1989.tb01576.x.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002C-48DA-F
Abstract
1. A method for determining free Ca2+-ions in the erythrocyte is described, using a commercially available ORION-Ca-electrode and calomel reference electrode assembly, where changes in free Ca2+-ion concentration upon addition of 0.01% digitonin could be measured. 2. The average value found for fresh cells from 20 healthy donors at 37°C (pH = 7.4) was 0.20 ± 0.04 μmol/L referred to a haematocrit of 10%. 3. Decrease of the simultaneously determined adenosinetriphosphate (ATP) concentration indicates that ATP is presumably needed to activate the Ca-ATPase. 4. In vitro addition of the calcium antagonists pentoxifylline and nifidepine, respectively, induced a normalization of the intraerythrocytic Ca2+-ion concentration after previous increase with the ion carrier ionophore A23187. 5. The advantages and possible clinical applications of this method are discussed.