Purification of High-Complexity Peptide Microarrays by Spatially Resolved Array 
Transfer to Gold-Coated Membranes

Schirwitz, Christopher; Löffler, Felix; Felgenhauer, Thomas; Stadler, Volker; Nesterov-Mueller, Alexander; Dahint, Reiner; Breitling, Frank; Bischoff, F. Ralf

doi:10.1002/adma.201203853

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Purification of High-Complexity Peptide Microarrays by Spatially Resolved Array Transfer to Gold-Coated Membranes

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Schirwitz, C., Löffler, F., Felgenhauer, T., Stadler, V., Nesterov-Mueller, A., Dahint, R., et al. (2013). Purification of High-Complexity Peptide Microarrays by Spatially Resolved Array Transfer to Gold-Coated Membranes. Advanced Materials, 25(11), 1598-1602. doi:10.1002/adma.201203853.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002C-524C-F

Abstract

A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an N-terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm(2) is demonstrated.