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Journal Article

Mapping metabolites from rough terrain: laser ablation electrospray ionization on non-flat samples

MPS-Authors
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Bartels,  Benjamin
Research Group Mass Spectrometry, MPI for Chemical Ecology, Max Planck Society;
IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society;

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Kulkarni,  Purva
Research Group Mass Spectrometry, MPI for Chemical Ecology, Max Planck Society;
IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society;

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Svatoš,  Aleš
Research Group Mass Spectrometry, MPI for Chemical Ecology, Max Planck Society;

External Resource
Fulltext (public)

MS182.pdf
(Publisher version), 2MB

Supplementary Material (public)

MS182s1.pdf
(Supplementary material), 115KB

Citation

Bartels, B., Kulkarni, P., Danz, N., Böcker, S., Saluz, H. P., & Svatoš, A. (2017). Mapping metabolites from rough terrain: laser ablation electrospray ionization on non-flat samples. RSC Advances, 7(15), 9045-9050. doi:10.1039/C6RA26854D.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002C-51D9-8
Abstract
Established laser-based ionization experiments require the surface of a sample to be as flat as possible to guarantee optimal laser focus. A laser ablation electrospray ionization (LAESI) source was custom-built to accommodate the topography of non-flat sample surfaces. Employing a confocal distance sensor, a height profile of the surface in question was recorded prior to the actual ionization experiment. The robustness of the system was evaluated by the metabolic profiling of radish (Raphanus sativus) leaves, chosen due to their pronounced surface features and known content of specialized metabolites. After the ionization experiments, light microscopy imaging was performed to evaluate ablation crater size and position. Reproducible ablation mark diameters of 69 7 mm in average have been achieved. Mass spectrometric imaging capability has been proven on R. sativus leaf samples as well.