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Cryptotomography: reconstructing 3D Fourier intensities from randomly oriented single-shot diffraction patterns

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Schlichting,  Ilme
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Shoeman,  Robert L.
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Lomb,  Lukas
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Loh, N.-T.-D., Bogan, M. J., Elser, V., Barty, A., Boutet, S., Bajt, S., et al. (2010). Cryptotomography: reconstructing 3D Fourier intensities from randomly oriented single-shot diffraction patterns. Physical Review Letters, 104(22): 225501, pp. 1-5. doi:10.1103/PhysRevLett.104.225501.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002C-5BAF-3
Abstract
We reconstructed the 3D Fourier intensity distribution of monodisperse prolate nanoparticles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast x-ray pulse intercepted individual particles of random, unmeasured orientations. This first experimental demonstration of cryptotomography extended the expansion-maximization-compression framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter. This work is an important step towards realizing single-shot diffraction imaging of single biomolecules.