Accurate delivery of single biomolecules by polyethylene glycol coated 
submicrometer pipettes

Park, Chong-Woo; Knemeyer, Jens-Peter; Marmé, Nicole; Möller, Martin; Spatz, Joachim P.; Wolfrum, Jürgen; Sauer, Markus

doi:10.1016/j.chemphys.2004.03.006

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Accurate delivery of single biomolecules by polyethylene glycol coated submicrometer pipettes

MPS-Authors

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Spatz, Joachim P.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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http://www.sciencedirect.com/science/article/pii/S0301010404001156/pdfft?md5=9433e8d19032dda74fb89fba5a779172&pid=1-s2.0-S0301010404001156-main.pdf
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http://dx.doi.org/10.1016/j.chemphys.2004.03.006
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引用

Park, C.-W., Knemeyer, J.-P., Marmé, N., Möller, M., Spatz, J. P., Wolfrum, J., & Sauer, M. (2004). Accurate delivery of single biomolecules by polyethylene glycol coated submicrometer pipettes. Chemical Physics, 301(1), 105-110. doi:10.1016/j.chemphys.2004.03.006.

引用: https://hdl.handle.net/11858/00-001M-0000-0028-34B1-1

要旨

There is a great interest to quantitatively manipulate, separate, and deliver low concentrations or even single biomolecules in submicrometer-sized channels. We report the use of cross-linked four-star polyethylene glycol (PEG) as a new coating technique for microchannels. PEG-coating efficiently reduces the electroosmotic flow (EOF) and non-specific adsorption to the glass walls. Our results demonstrate that individual fluorescently labeled hydrophobic cell adhesion proteins (fibronectin molecules) can be drawn through PEG-coated submicrometer pipettes in aqueous solution by electrokinetic forces without the addition of detergents or other additives which potentially deteriorate the activity and specificity of biomolecules.