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Calcium-independent activation of an allosteric network in Langerin by heparin oligosaccharides

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Hanske,  Jonas
Christoph Rademacher, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Wawrzinek,  Robert
Christoph Rademacher, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Geissner,  Andreas
Chakkumal Anish, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Wamhoff,  Eike-Christian
Christoph Rademacher, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Sellrie,  Katrin
Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Schmidt,  Henrik
Christoph Rademacher, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Seeberger,  Peter H.
Peter H. Seeberger - Vaccine Development, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Rademacher,  Christoph
Christoph Rademacher, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Citation

Hanske, J., Wawrzinek, R., Geissner, A., Wamhoff, E.-C., Sellrie, K., Schmidt, H., et al. (2017). Calcium-independent activation of an allosteric network in Langerin by heparin oligosaccharides. ChemBioChem: A European Journal of Chemical Biology, 18(13), 1183-1187. doi:10.1002/cbic.201700027.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002C-7EED-3
Abstract
The C-type lectin receptor Langerin is a glycan binding protein that serves as an uptake receptor on Langerhans cells and is essential for the formation of Birbeck granules. Whereas most of Langerin´s ligands are recognized via a canonical Ca2+-dependent binding site, interactions with heparins have been proposed to make additional contacts to a secondary, Ca2+-independent site. Glycan array screening and biomolecular NMR spectroscopy were employed to investigate the molecular mechanism of these interactions. We observed that binding of heparin hexasaccharides to a secondary site does not require the presence of Ca2+ and activates a previously identified intradomain allosteric network of Langerin