Interaction of DNA hairpin loops and a complementary strand by a triplet of 
base pairs.

Baumann, U.; Frank, R.; Blöcker, H.

doi:10.1016/S0006-291X(88)80971-9

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Interaction of DNA hairpin loops and a complementary strand by a triplet of base pairs.

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Baumann, U.
Research Group of Molecular Organized Systems, MPI for biophysical chemistry, Max Planck Society;

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Citation

Baumann, U., Frank, R., & Blöcker, H. (1988). Interaction of DNA hairpin loops and a complementary strand by a triplet of base pairs. Biochemical and Biophysical Research Communications, 157(3), 986-991. doi:10.1016/S0006-291X(88)80971-9.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-2B20-1

Abstract

Hypothesis of non-enzymatic recognition of primordial tRNA and mRNA precursors is experimentally approached. DNA hairpins containing a different number of deoxyguanosine residues in the loop are analyzed for their binding ability to a chemically fixed single-strand of oligo(dC). In presence of small Mg2+ concentration a hairpin with five dG residues in the loop is adsorbed to affinity matrix. Comparison of elution temperatures of hairpin oligonucleotides with those of single-stranded oligoguanylic acids with length of the loop indicates, that smallest loop able to bind forms a triplet of base pairs.