Affinity labelling and identification of the high-affinity choline carrier from 
synaptic membranes of Torpedo electromotor nerve terminals with [3H]choline 
mustard.

Rylett, R.J.

doi:10.1111/j.1471-4159.1988.tb01182.x

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Affinity labelling and identification of the high-affinity choline carrier from synaptic membranes of Torpedo electromotor nerve terminals with [3H]choline mustard.

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Rylett, R.J.
Abteilung Neurochemie, MPI for biophysical chemistry, Max Planck Society;

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Citation

Rylett, R. (1988). Affinity labelling and identification of the high-affinity choline carrier from synaptic membranes of Torpedo electromotor nerve terminals with [3H]choline mustard. Journal of Neurochemistry, 51(6), 1942-1945. doi:10.1111/j.1471-4159.1988.tb01182.x.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-2E57-2

Abstract

The physiological mechanisms regulating activity of the sodium-dependent, high-affinity choline transporter and the molecular events in the translocation process remain unclear; the protein has not been purified or characterized biochemically. In the present study, [3H]choline mustard aziridinium ion ([3H]ChM Az), a nitrogen mustard analogue of choline, bound irreversibly to presynaptic plasma membranes from Torpedo electric organ in a hemicholinium-sensitive, and sodium-, time-, and temperature-dependent manner. Specific binding of this ligand was greatest when it was incubated with membranes in the presence of sodium at 30°C. Separation of the 3H-labelled membrane proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that most of the radiolabel was associated with a polypeptide of apparent molecular mass of ∼42,000 daltons; labelling of this species was abolished in membranes incubated with ligand in the presence of HC-3. Two other 3H-labelled polypeptides were detected, with apparent molecular masses of ∼58,000 and 90,000 daltons; radiolabelling of the former was also HC-3 sensitive. [3H]ChM Az may be a useful affinity ligand in the purification of the choline carrier from cholinergic neurons.