User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse




Journal Article

Intracellular calcium release mediated by two muscarinic receptor subtypes.


Neher,  E.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

External Ressource
No external resources are shared
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available

Neher, E., Marty, A., Fukuda, K., Kubo, T., & Numa, S. (1988). Intracellular calcium release mediated by two muscarinic receptor subtypes. FEBS Letters, 240(1-2), 88-94. doi:10.1016/0014-5793(88)80345-4.

Cite as: http://hdl.handle.net/11858/00-001M-0000-002D-2EF2-2
Four subtypes of muscarinic acetylcholine receptor (mAChR) were stably expressed in neuroblastoma-glioma hybrid cells (NG108-15). By combining fluorescent indicator dye (fura-2) studies with electrophysiological measurements it is shown that stimulation of mAChR I and mAChR III readily leads to release of calcium from intracellular stores and to associated conductance changes, whereas stimulation of mAChR II and mAChR IV exerts no such effect. Dose-response curves describing the amplitude or the delay of the calcium rise induced by acetylcholine suggest that the apparent affinity of mAChR III for its agonist is higher by about one order of magnitude than that of mAChR I. Ionic substitution experiments and current fluctuation analysis indicate that calcium activates a K+-specific conductance of ‘small’ single-channel amplitude similar to the SK type [1]. Furthermore, an outward current (M current) suppressed by activation of mAChR I and mAChR III has a single-channel amplitude corresponding to a conductance of approximately 3 pS.