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Journal Article

Kontraktionszyklus und Interaktion zwischen Aktin und l-Myosin unter der Wirkung spezifischer Interaktions-Inhibitoren


Bárány,  M.
Max Planck Institute for Medical Research, Max Planck Society;


Jaisle,  F.
Max Planck Institute for Medical Research, Max Planck Society;

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Bárány, M., & Jaisle, F. (1960). Kontraktionszyklus und Interaktion zwischen Aktin und l-Myosin unter der Wirkung spezifischer Interaktions-Inhibitoren. Biochimica et Biophysica Acta (BBA), 41(2), 192-203. doi:10.1016/0006-3002(60)90002-0.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-319D-2
Contraction cycle and inhibitors of actin-L-myosin interaction 1. 1. The term interaction inhibitor describes such substances that never affect L-myosin induced ATP splitting but that, when in the presence of ATP, do inhibit the enzymic interaction between actin and L-myosin, i.e., they convert the Mg-activated actomyosin ATPase reversibly into L-myosin ATPase. 2. 2. Examples of interaction inhibitors are the relaxing factor, discovered by Marsh and Bendall, some polycations, polysulfonates, EDTA, and SCN- in low concentrations. 3. 3. It is shown for polythensulfonate, EDTA, Fuadin, and SCN- and confirmed for the relaxing factor that in the presence of ATP these substances inhibit reversibly the association of actin and L-myosin as well as their chemical interaction. 4. 4. For the fibrils in the water-glycerol extracted fibers this is demonstrated by measuring the resistance to stretching. 5. 5. For gels from natural actomyosin the same conclusion can be drawn, because the actin-component can be almost completely extracted from these gels by an ATP-containing polyethensulfonate solution. 6. 6. Contracted water-glycerol extracted fibers relax as soon as the actin and L-myosin filaments dissociate through the action of ATP and an interaction inhibitor. 7. 7. The dissociation of actin and L-myosin disappears if either the concentration of ATP or that of the interaction inhibitor is sufficiently lowered. 8. 8. In both cases contraction and ATP splitting by Mg-activated actomyosin ATPase occurs anew along with the reappearing association. 9. 9. In the presence of ATP and an interaction inhibitor relaxation even occurs if the Mg-activated actomysin ATPase is converted into the highly effective Ca-activated L-myosin ATPase instead of into the slightly effective Mg-activated L-myosin ATPase. Hence contraction is only produced when the mechanism of ATP splitting is based on the interaction between actin and L-myosin. Contraction is independent of ATP-splitting by L-myosin ATPase.