English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Kontraktionszyklus und Interaktion zwischen Aktin und l-Myosin unter der Wirkung spezifischer Interaktions-Inhibitoren

MPS-Authors
/persons/resource/persons205217

Bárány,  M.
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons205209

Jaisle,  F.
Max Planck Institute for Medical Research, Max Planck Society;

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Bárány, M., & Jaisle, F. (1960). Kontraktionszyklus und Interaktion zwischen Aktin und l-Myosin unter der Wirkung spezifischer Interaktions-Inhibitoren. Biochimica et Biophysica Acta (BBA), 41(2), 192-203. doi:10.1016/0006-3002(60)90002-0.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-319D-2
Abstract
Contraction cycle and inhibitors of actin-L-myosin interaction 1. 1. The term interaction inhibitor describes such substances that never affect L-myosin induced ATP splitting but that, when in the presence of ATP, do inhibit the enzymic interaction between actin and L-myosin, i.e., they convert the Mg-activated actomyosin ATPase reversibly into L-myosin ATPase. 2. 2. Examples of interaction inhibitors are the relaxing factor, discovered by Marsh and Bendall, some polycations, polysulfonates, EDTA, and SCN- in low concentrations. 3. 3. It is shown for polythensulfonate, EDTA, Fuadin, and SCN- and confirmed for the relaxing factor that in the presence of ATP these substances inhibit reversibly the association of actin and L-myosin as well as their chemical interaction. 4. 4. For the fibrils in the water-glycerol extracted fibers this is demonstrated by measuring the resistance to stretching. 5. 5. For gels from natural actomyosin the same conclusion can be drawn, because the actin-component can be almost completely extracted from these gels by an ATP-containing polyethensulfonate solution. 6. 6. Contracted water-glycerol extracted fibers relax as soon as the actin and L-myosin filaments dissociate through the action of ATP and an interaction inhibitor. 7. 7. The dissociation of actin and L-myosin disappears if either the concentration of ATP or that of the interaction inhibitor is sufficiently lowered. 8. 8. In both cases contraction and ATP splitting by Mg-activated actomyosin ATPase occurs anew along with the reappearing association. 9. 9. In the presence of ATP and an interaction inhibitor relaxation even occurs if the Mg-activated actomysin ATPase is converted into the highly effective Ca-activated L-myosin ATPase instead of into the slightly effective Mg-activated L-myosin ATPase. Hence contraction is only produced when the mechanism of ATP splitting is based on the interaction between actin and L-myosin. Contraction is independent of ATP-splitting by L-myosin ATPase.