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Journal Article

Double-flow focused liquid injector for efficient serial femtosecond crystallography

MPS-Authors
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Paulraj,  Lourdu Xavier
International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany;
Department of Physics, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany;

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https://doi.org/10.1038/srep44628
(Publisher version)

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srep44628.pdf
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Citation

Oberthuer, D., Knoška, J., Wiedorn, M. O., Beyerlein, K. R., Bushnell, D. A., Kovaleva, E. G., et al. (2017). Double-flow focused liquid injector for efficient serial femtosecond crystallography. Scientific Reports, 7: 44628 (2017). doi:10.1038/srep44628.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-5498-1
Abstract
Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.