English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Small-angle scattering of apoferritin in solution

MPS-Authors
/persons/resource/persons197081

Bielig,  Hans-Joachim
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons205430

Kratky,  O.
Max Planck Institute for Medical Research, Max Planck Society;

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Bielig, H.-J., Kratky, O., Rohns, G., & Wawra, H. (1966). Small-angle scattering of apoferritin in solution. Biochimica et Biophysica Acta: BBA, 112(1), 110-118. doi:10.1016/S0926-6585(96)90013-8.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-407C-4
Abstract
Native, undried, crystalline apoferritin, prepared from ferritin, was studied in aqueous solution (0.173–4.65%) by the X-ray small-angle method. Fourier transformation of the intensity distribution of the scattering curve of the apoferritin showed a density distribution corresponding to a hollow sphere with ri′ = 35.5 Å and ra′ = 62.7Å. The molecular weight of apoferritin was estimated as 462000 from measurements of the absolute intensity of the scattering. The molecular volume of the hydrated protein shell, derived from the invariant, is 82.5·104 Å3 and derived from the dimensions of the hollow sphere, is 84.0·104 Å3. The hydration was calculated from comparison with the volume in the non-hydrated state (57.4·104 Å3), as 0.327 g water per g protein.