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Glycan Fingerprinting via Cold-Ion Infrared Spectroscopy

MPS-Authors
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Mucha,  Eike
Molecular Physics, Fritz Haber Institute, Max Planck Society;
Institut für Chemie und Biochemie der Freien Universität Berlin;

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Gonzalez Florez,  Ana Isabel
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Marianski,  Mateusz
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Thomas,  Daniel
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Hoffmann,  Waldemar
Molecular Physics, Fritz Haber Institute, Max Planck Society;
Institut für Chemie und Biochemie der Freien Universität Berlin;

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Hahm,  Heung Sik
Peter H. Seeberger - Automated Systems, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Gewinner,  Sandy
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Schöllkopf,  Wieland
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Seeberger,  Peter H.
Peter H. Seeberger - Automated Systems, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;
Institut für Chemie und Biochemie der Freien Universität Berlin;

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Helden,  Gert von
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Pagel,  Kevin
Molecular Physics, Fritz Haber Institute, Max Planck Society;
Institut für Chemie und Biochemie der Freien Universität Berlin;

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Citation

Mucha, E., Gonzalez Florez, A. I., Marianski, M., Thomas, D., Hoffmann, W., Struwe, W. B., et al. (2017). Glycan Fingerprinting via Cold-Ion Infrared Spectroscopy. Angewandte Chemie International Edition, 56(37), 11248-11251. doi:10.1002/anie.201702896.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002D-4DB3-9
Abstract
The diversity of stereochemical isomers present in glycans and glycoconjugates poses a formidable challenge for comprehensive structural analysis. Typically, sophisticated mass spectrometry (MS)- based techniques are used in combination with chromatography or ion mobility separation. However, coexisting structurally similar isomers often render an unambiguous identification impossible. Other powerful techniques such as gas-phase infrared (IR) spectroscopy have been limited to smaller glycans, as conformational flexibility and thermal activation during the measurement result in poor spectral resolution. Here, we show that this limitation can be overcome using cold-ion spectroscopy. The vibrational fingerprints of cold oligosaccharide ions exhibit a wealth of well-resolved absorption features that are diagnostic for minute structural variations. The unprecedented resolution of cold-ion spectroscopy coupled with tandem MS may render this the key technology to unravel complex glycomes.