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Large-Scale Mitotic Cell Synchronization

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Dulla,  Kalyan
Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Dulla, K., & Margalef, A. S. (2017). Large-Scale Mitotic Cell Synchronization. In CELL CYCLE SYNCHRONIZATION: METHODS AND PROTOCOLS, 2ND EDITION (pp. 65-74). 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA: HUMANA PRESS INC.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002D-CDC5-7
Abstract
Understanding cell growth and cell division involves the study of regulatory events that occur in a cell cycle phase-dependent manner. Studies analyzing cell cycle regulatory mechanisms and cell cycle progression invariably require synchronization of cell populations at specific cell cycle stages. Several methods have been established to synchronize cells, including serum deprivation, contact inhibition, centrifugal elutriation, and drug-dependent synchronization. Despite potential adverse cellular consequences of synchronizing cells by pharmacological agents, drug-dependent methods can be advantageous when studying later cell cycle events to ensure specific enrichment at selected mitotic stages. This chapter describes protocols used in our laboratory for isolating mitotic mammalian cells in a large-scale manner. In particular, we discuss the technical aspects of adherent or suspension cell isolation, the methods necessary to enrich cells at different mitotic stages and the optimized culture conditions.