Endonuclease I-deficient and ribonuclease I-deficient Escherichia coli mutants

Dürwald, Hildegard; Hoffman-Berling, Hartmut

doi:10.1016/0022-2836(68)90257-X

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Endonuclease I-deficient and ribonuclease I-deficient Escherichia coli mutants

MPS-Authors

/persons/resource/persons205356

Dürwald, Hildegard
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons203965

Hoffman-Berling, Hartmut
Max Planck Institute for Medical Research, Max Planck Society;

External Resource

http://www.sciencedirect.com/science/article/pii/002228366890257X/pdf?md5=286a39dae551035d3527ee750de6381b&pid=1-s2.0-002228366890257X-main.pdf
(Any fulltext)

https://doi.org/10.1016/0022-2836(68)90257-X
(Any fulltext)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Dürwald, H., & Hoffman-Berling, H. (1968). Endonuclease I-deficient and ribonuclease I-deficient Escherichia coli mutants. Journal of Molecular Biology (London), 34(2), 331-346. doi:10.1016/0022-2836(68)90257-X.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-542A-A

Abstract

A method has been developed to select for Escherichia coli mutants which lack certain nucleases. Using this method, two classes of mutants have been isolated: mutants deficient in endonuclease I, and mutants deficient in RNase I. Extracts from endonuclease I mutants had 0.1 to 0.3% of the RNA-sensitive wild-type endonuclease and, using single-stranded DNA, no RNA-resistant endonuclease. The location of the endonuclease I mutation on the bacterial genetic map was shown to be near st on the side opposite to mtl. Extracts from RNase I mutants had 0.1% of the magnesium-independent wild-type RNase or lower activity. Mutants deficient in endonuclease I or RNase I were essentially identical to wild-type with respect to their general biological properties.