Initiation and termination events in double stranded ϕx-DNA replication

Knippers, Rolf; Müller-Wecker, Hildegard

doi:10.1111/j.1432-1033.1970.tb00989.x

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Initiation and termination events in double stranded ϕx-DNA replication

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Knippers, Rolf
Max Planck Institute for Medical Research, Max Planck Society;

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Müller-Wecker, Hildegard
Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Knippers, R., & Müller-Wecker, H. (1970). Initiation and termination events in double stranded ϕx-DNA replication. The FEBS Journal, 15(1), 146-154. doi:10.1111/j.1432-1033.1970.tb00989.x.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-60F6-0

Abstract

The experiments reported in this paper lead to the following conclusions:

1An endonucleolytic activity is associated with the “replication site” in Escherichia coli cells; this endonucleolytic activity attacks the complementary strand of the ϕ x replicative form DNA only.
2The viral strand of the ϕ x-replicative form DNA remains circularly closed during the replication cycle;
3In the immediate replication product both composing strands are linear; in circularization no strand is prefered;
4A replication product with both strands closed is transiently found in a structural state which can be, under our experimental conditions, only partially denatured by alkali. Subsequently it is transformed to a typical supercoiled RFI structure.