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Book Chapter

Recent Advances in Directed Evolution of Stereoselective Enzymes


Reetz,  Manfred T.
Philipps-Universität Marburg, Fachbereich Chemie;
Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society;

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Reetz, M. T. (2017). Recent Advances in Directed Evolution of Stereoselective Enzymes. In M. Alcalde (Ed.), Directed Enzyme Evolution: Advances and Applications (pp. 69-99). Cham: Springer. doi:10.1007/978-3-319-50413-1_3.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-71BC-7
Directed evolution of enzymes provides a prolific source of biocatalysts for asymmetric reactions in organic chemistry and biotechnology. Nowadays, the real challenge in this research area is the development of mutagenesis methods and strategies which ensure the formation of small and highest-quality mutant libraries requiring minimal screening effort. This chapter constitutes a critical analysis of recent developments. Saturation mutagenesis at sites lining the binding pocket using highly reduced amino acid alphabets has emerged as the superior approach for evolving stereoselectivity.