Help Privacy Policy Disclaimer
  Advanced SearchBrowse




Journal Article

Suche nach einem Metaboliten bei Vergiftung mit Desmethylphalloin (DMP)


Puchinger,  Herwig
Max Planck Institute for Medical Research, Max Planck Society;


Wieland,  Theodor
Max Planck Institute for Medical Research, Max Planck Society;

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available

Puchinger, H., & Wieland, T. (1969). Suche nach einem Metaboliten bei Vergiftung mit Desmethylphalloin (DMP). European Journal of Biochemistry, 11(1), 1-6. doi:10.1111/j.1432-1033.1969.tb00731.x.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-77FF-1
Various observations cited at the beginning of this paper and in the Discussion suggested that phalloidine, a poisonous constituent of the green mushroom Amanita phalloides, would not be toxic by itself, but would be rendered toxic previously in the liver. Using a tritiated toxic derivative of phalloidine ([3H]desmethylphalloin) we found that: (a) in rats, 60% of the administered substance are excreted unchanged in the urine within the first day. A second radioactive substance of low concentration, moving more slowly in paper chromatography, turned out as a product of the self-degradation of [3H]desmethylphalloin; (b) respectively 98% and 99,9% of the radioactivity were extracted with methanol from homogenates of livers of rats or mice which had received [3H]desmethylphalloin 1 to 2 hours before. Aqueous homogenates of liver of poisened rats also contained radioactivity firmly bound to a high molecular weight substance, as shown by gel chromatography. The radioactive substance was totally removed from the carrier, presumably ribosomal material, by adding methanol, and was identified as [3H]desmethylphalloin; (c) [3H]desmethylphalloin was not metabolized on incubation with oxygen and rat liver ribosomes in a NADPH-regenerating system. A radioactive substance extracted from liver microsomes of a poisoned rat also proved to be original toxin. As a result of these experiments it can be concluded that [3H]desmethylphalloin and most probably also phalloidine are not metabolized in the livers of rats and mice.