The enzymatic synthesis of thiophosphate analogs of nucleotides

Goody, Roger S.; Eckstein, F.; Schirmer, R. Heiner

doi:10.1016/0005-2744(72)90016-2

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

The enzymatic synthesis of thiophosphate analogs of nucleotides

MPS-Authors

/persons/resource/persons98693

Goody, Roger S.
Abt. III: Physikalische Biochemie, Max Planck Institute of Molecular Physiology, Max Planck Society;

/persons/resource/persons206521

Eckstein, F.
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons205497

Schirmer, R. Heiner
Max Planck Institute for Medical Research, Max Planck Society;

External Resource

http://www.sciencedirect.com/science/article/pii/0005274472900162/pdf?md5=4185df54639fe12564d04516e6ff6ee7&pid=1-s2.0-0005274472900162-main.pdf
(Any fulltext)

https://doi.org/10.1016/0005-2744(72)90016-2
(Any fulltext)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Goody, R. S., Eckstein, F., & Schirmer, R. H. (1972). The enzymatic synthesis of thiophosphate analogs of nucleotides. Biochimica et Biophysica Acta: BBA, 276(1), 155-161. doi:10.1016/0005-2744(72)90016-2.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-8A5E-F

Abstract

The enzymatic transfer of the thiophosphate group from a nucleoside 5′-O-(3-thiotriphosphate) to a nucleoside 5′-diphosphate with the aid of nucleosidediphosphate kinase (ATP: nucleosidediphosphate phosphotransferase, EC 2.7.4.6) is described. This method can be used for the synthesis of radioactively labelled nucleoside 5′-O-(3-thiotriphosphates). Thiophosphate transfer can also be achieved using adenylate kinase (ATP: AMP phosphotransferase, EC 2.7.4.3).