English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Video-rate volumetric functional imaging of the brain at synaptic resolution

MPS-Authors
/persons/resource/persons188171

Seelig,  Johannes Dominik
Max Planck Research Group Neural Circuits, Center of Advanced European Studies and Research (caesar), Max Planck Society;

External Resource
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Lu, R., Sun, W., Liang, Y., Kerlin, A., Bierfeld, J., Seelig, J. D., et al. (2017). Video-rate volumetric functional imaging of the brain at synaptic resolution. Nature Neuroscience, 20(4), 620-628. doi:10.1038/nn.4516.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-8C1F-D
Abstract
Neurons and neural networks often extend hundreds of micrometers in three dimensions. Capturing the calcium transients associated with their activity requires volume imaging methods with subsecond temporal resolution. Such speed is a challenge for conventional two-photon laser-scanning microscopy, because it depends on serial focal scanning in 3D and indicators with limited brightness. Here we present an optical module that is easily integrated into standard two-photon laser-scanning microscopes to generate an axially elongated Bessel focus, which when scanned in 2D turns frame rate into volume rate. We demonstrated the power of this approach in enabling discoveries for neurobiology by imaging the calcium dynamics of volumes of neurons and synapses in fruit flies, zebrafish larvae, mice and ferrets in vivo. Calcium signals in objects as small as dendritic spines could be resolved at video rates, provided that the samples were sparsely labeled to limit overlap in their axially projected images.