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Abstract

1Adenylate kinase (ATP:AMP phosphotransferase) has been purified 490-fold from porcine muscle with a final yield of 60 mg/kg muscle. 2The amino-acid composition is Asp11, Asn2, Thr14, Ser11, Glu19, Gln6, Pro6, Gly19, Ala8, Cys2, Val17, Met6, Ile9, Leu18. Tyr7, Phe5, Lys21, His2, Arg11. 3The protein molecule is a single polypeptide chain of 194 amino-acid residues with an acetyl-methionine at the N-terminus and a lysine residue at the C-terminus. 4Cyanogen bromide cleavage of carboxymethylated adenylate kinase yielded six fragments which were further degraded by using trypsin, chymotrypsin, thermolysin, subtilisin or α-protease. Sequence data on the resulting peptides are summarized in the present report, full details are given in a supplementary paper which has been deposited at CNRS from where copies can be obtained. 5The primary structure of porcine adenylate kinase is: Ac-Met-Glu-Glu-Lys-Leu-Lys-Lys-Ser-Lys-Ile10-Ile-Phe-Val-Val-Gly-Gly-Pro-Gly - Ser-Gly20-Lys-Gly-Thr-Gln-Cys-Glu-Lys-Ile-Val-Gln30-Lys-Tyr-Gly-Tyr-Thr-His-Leu-Ser-Thr-Gly40-Asp-Leu-Leu-Arg-Ala-Glu-Val-Ser-Ser-Gly50-Ser-Ala-Arg-Gly-Lys-Met-Leu-Ser-Glu-Ile60-Met-Glu-Lys-Gly-Gln-Leu-Val-Pro-Leu-Glu70-Thr-Val-Leu-Asp-Met-Leu-Arg-Asp-Ala-Met80-Val-Ala-Lys-Val-Asp-Thr-Ser-Lys-Gly-Phe90-Leu-Ile-Asp-Gly-Tyr-Pro-Arg-Glu-Val-Lys100-Gln-Gly-Glu-Glu-Phe-Glu-Arg-Lys-Ile-Gly110-Gln-Pro-Thr-Leu-Leu-Leu-Tyr-Val-Asp120-Ala-Gly-Pro-Glu-Thr-Met-Thr-Lys-Arg-Leu-Leu130-Lys-Arg-Gly-Glu-Thr-Ser-Gly-Arg-Val-Asp140-Asp-Asn-Glu-Glu-Thr-Ile-Lys-Lys-Arg-Leu150-Glu-Thr-Tyr-Tyr-Lys-Ala-Thr-Glu-Pro-Val160-Ile-Ala-Phe-Tyr-Glu-Lys-Arg-Gly-Ile-Val170-Arg-Lys-Val-Asn-Ala-Glu-Gly-Ser-Val-Asp180-Asp-Val-Phe-Ser-Gln-Val-Cys-Thr-His-Leu190-Asp-Thr-Leu-Lys.