Presynaptic glycine receptors on hippocampal mossy fibers

Kubota, H.; Alle, H.; Betz, H.; Geiger, J. R. P.

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Presynaptic glycine receptors on hippocampal mossy fibers

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Kubota, H.
Neurochemistry Department, Max Planck Institute for Brain Research, Max Planck Society;

Alle, H.
Max Planck Society;

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Betz, H.
Neurochemistry Department, Max Planck Institute for Brain Research, Max Planck Society;

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Geiger, J. R. P.
Neurochemistry Department, Max Planck Institute for Brain Research, Max Planck Society;

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Kubota, H., Alle, H., Betz, H., & Geiger, J. R. P. (2010). Presynaptic glycine receptors on hippocampal mossy fibers. Biochemical and Biophysical Research Communications, 393(4), 587-591.

Cite as: http://hdl.handle.net/11858/00-001M-0000-002E-1B37-1

Abstract

Presynaptic glycine receptors (GlyRs) have been implicated in the regulation of glutamatergic synaptic transmission. Here, we characterized presynaptic GlyR-mediated currents by patch-clamp recording from mossy fiber boutons (MFBs) in rat hippocampal slices. In MFBs, focal puff-application of glycine-evoked chloride currents that were blocked by the GlyR antagonist strychnine. Their amplitudes declined substantially during postnatal development, from a mean conductance per MFB of similar to 600 pS in young to similar to 130 pS in adult animals. Single-channel analysis revealed multiple conductance states between 20 and similar to 120 pS, consistent with expression of both homo- and hetero-oligomeric GlyRs. Accordingly, estimated GlyRs densities varied between 8-17 per young, and 1-3 per adult, MFB. Our results demonstrate that functional presynaptic GlyRs are present on hippocampal mossy fiber terminals and suggest a role of these receptors in the regulation of glutamate release during the development of the mossy fiber - CA3 synapse. (C) 2010 Elsevier Inc. All rights reserved.