Deutsch
 
Benutzerhandbuch Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Revision and reannotation of the Halomonas elongata DSM 2581(T) genome.

MPG-Autoren
/persons/resource/persons78503

Pfeiffer,  Friedhelm
Habermann, Bianca / Computational Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons209133

Zamora-Lagos,  Maria-A.
Habermann, Bianca / Computational Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons101406

Habermann,  Bianca
Habermann, Bianca / Computational Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78468

Oesterhelt,  Dieter
Oesterhelt, Dieter / Membrane Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

Externe Ressourcen
Es sind keine Externen Ressourcen verfügbar
Volltexte (frei zugänglich)

Pfeiffer_mbo3465.pdf
(Verlagsversion), 315KB

Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Pfeiffer, F., Bagyan, I., Alfaro-Espinoza, G., Zamora-Lagos, M.-A., Habermann, B., Marin-Sanguino, A., et al. (2017). Revision and reannotation of the Halomonas elongata DSM 2581(T) genome. MicrobiologyOpen, e465. doi:10.1002/mbo3.465.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-002D-E59E-3
Zusammenfassung
The genome of the Halomonas elongata type strain DSM 2581, an industrial producer, was reevaluated using the Illumina HiSeq2500 technology. To resolve duplication-associated ambiguities, PCR products were generated and sequenced. Outside of duplications, 72 sequence corrections were required, of which 24 were point mutations and 48 were indels of one or few bases. Most of these were associated with polynucleotide stretches (poly-T stretch overestimated in 19 cases, poly-C underestimated in 15 cases). These problems may be attributed to using 454 technology for original genome sequencing. On average, the original genome sequence had only one error in 56kb. There were 23 frameshift error corrections in the 29 protein-coding genes affected by sequence revision. The genome has been subjected to major reannotation in order to substantially increase the annotation quality. 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.