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Journal Article

Cell-permeant large stokes shift dyes for transfection-free multicolor nanoscopy.

MPS-Authors
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Butkevich,  A.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Lukinavicius,  G.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

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D'Este,  E.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Hell,  S. W.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Citation

Butkevich, A., Lukinavicius, G., D'Este, E., & Hell, S. W. (2017). Cell-permeant large stokes shift dyes for transfection-free multicolor nanoscopy. Journal of the American Chemical Society, 139(36), 12378-12381. doi:10.1021/jacs.7b06412.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002D-E252-5
Abstract
We designed cell-permeant red-emitting fluorescent dye labels with >140 nm Stokes shifts based on 9-iminoanthrone, 9-imino-10-silaxanthone, and 9-imino-10-germaxanthone fluorophores. The corresponding probes selectively targeting mitochondria, lysosomes, and F-actin demonstrate low toxicity and enable stimulated emission depletion (STED) nanoscopy in neurons, human fibroblasts, U2OS, and HeLa cells. In combination with known small Stokes shift dyes, our probes allow live-cell three-color STED nanoscopy of endogenous targets on popular setups with 775 nm STED wavelength.