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Adsorption of cytochrome c to phospholipid monolayers studied by reflection spectroscopy.

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Kozarac,  Z.
Research Group of Molecular Organized Systems, MPI for biophysical chemistry, Max Planck Society;

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Dhathathreyan,  A.
Research Group of Molecular Organized Systems, MPI for biophysical chemistry, Max Planck Society;

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Möbius,  D.
Research Group of Molecular Organized Systems, MPI for biophysical chemistry, Max Planck Society;

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引用

Kozarac, Z., Dhathathreyan, A., & Möbius, D. (1988). Adsorption of cytochrome c to phospholipid monolayers studied by reflection spectroscopy. FEBs Letters, 229(2), 372-376. doi:10.1016/0014-5793(88)81159-1.


引用: https://hdl.handle.net/11858/00-001M-0000-002D-E7F9-3
要旨
The uptake of cytochrome c by charged and neutral lipid monolayers was studied by using reflection spectroscopy. The method was shown to be a very sensitive and useful technique in studies of lipid-protein interactions. It was found that cytochrome c is preferentially bound to monolayers of negatively charged monolayers in the solid phase. Polarized light under oblique incidence was used to determine the average orientation of chromophores in cytochrome c bound to lipid monolayer. The transition moments of chromophore are oriented parallel to the monolayer plane.