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Zeitschriftenartikel

Thermophilic archaea activate butan via alkyl-coenzyme M formation

MPG-Autoren

Laso-Perez,  Rafael
Max Planck Society;

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Wegener,  Gunter
HGF MPG Joint Research Group for Deep Sea Ecology & Technology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Knittel,  Katrin
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Widdel,  Friedrich
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Harding,  Katie Jean
Department of Biogeochemistry, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Krukenberg,  Viola
HGF MPG Joint Research Group for Deep Sea Ecology & Technology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Meier,  Dimitri
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Richter,  Michael
Microbial Genomics Group, Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Tegetmeyer,  Halina E.
HGF MPG Joint Research Group for Deep Sea Ecology & Technology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Musat,  Florin
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Zitation

Laso-Perez, R., Wegener, G., Knittel, K., Widdel, F., Harding, K. J., Krukenberg, V., et al. (2016). Thermophilic archaea activate butan via alkyl-coenzyme M formation. Nature, 539: 1, pp. 396-401.


Zitierlink: https://hdl.handle.net/21.11116/0000-0001-C247-4
Zusammenfassung
The anaerobic formation and oxidation of methane involve unique enzymatic mechanisms and cofactors, all of which are believed to be specific for C1-compounds. Here we show that an anaerobic thermophilic enrichment culture composed of dense consortia of archaea and bacteria apparently uses partly similar pathways to oxidize the C4 hydrocarbon butane. The archaea, proposed genus ‘Candidatus Syntrophoarchaeum’, show the characteristic autofluorescence of methanogens, and contain highly expressed genes encoding enzymes similar to methyl-coenzyme M reductase. We detect butyl-coenzyme M, indicating archaeal butane activation analogous to the first step in anaerobic methane oxidation. In addition, Ca. Syntrophoarchaeum expresses the genes encoding β-oxidation enzymes, carbon monoxide dehydrogenase and reversible C1 methanogenesis enzymes. This allows for the complete oxidation of butane. Reducing equivalents are seemingly channelled to HotSeep-1, a thermophilic sulfate-reducing partner bacterium known from the anaerobic oxidation of methane. Genes encoding 16S rRNA and methyl-coenzyme M reductase similar to those identifying Ca. Syntrophoarchaeum were repeatedly retrieved from marine subsurface sediments, suggesting that the presented activation mechanism is naturally widespread in the anaerobic oxidation of short-chain hydrocarbons.