Metagenomes: 23S Sequences. Encyclopedia of Metagenomics: Genes, Genomes and 
Metagenomes: Basics, Methods, Databases and Tools

Yilmaz, Pelin; Glöckner, Frank Oliver

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Metagenomes: 23S Sequences. Encyclopedia of Metagenomics: Genes, Genomes and Metagenomes: Basics, Methods, Databases and Tools

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Yilmaz, Pelin
Microbial Genomics Group, Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Glöckner, Frank Oliver
Microbial Genomics Group, Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Citation

Yilmaz, P., & Glöckner, F. O. (2015). Metagenomes: 23S Sequences. Encyclopedia of Metagenomics: Genes, Genomes and Metagenomes: Basics, Methods, Databases and Tools. In K. E. Nelson (Ed.), Encyclopedia of Metagenomics (pp. 396-402). New York: Springer.

Cite as: https://hdl.handle.net/21.11116/0000-0001-C4A5-7

Abstract

As an evolutionary marker, 23S ribosomal RNA (rRNA) offers more diagnostic sequence stretches and greater sequence variation than 16S rRNA. The main drawback of using 23S rRNA as a phylogenetic marker is that it is still not as widely used. In a survey of 23S rRNA gene sequences found in metagenomic datasets, the Global Ocean Sampling (GOS) metagenome revealed that 23S rRNA gene sequences are twice as abundant as 16S rRNA gene fragments, with 23S rRNA gene fragments being generally about 100 bp longer.