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Physiologische Charakterisierung von Mutanten im Monoterpenstoffwechsel von Castellaniella defragrans

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Mahler,  Linda Gloria
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Citation

Mahler, L. G. (2013). Physiologische Charakterisierung von Mutanten im Monoterpenstoffwechsel von Castellaniella defragrans. Bachelor Thesis, Beuth Hochschule für Technik Berlin, Berlin / Germany.


Cite as: https://hdl.handle.net/21.11116/0000-0001-C72F-B
Abstract
The facultative anaerobic betaproteobacterium Castellaniella defragrans is able to metabolise monoterpenes under anoxic conditions. Proteom studies showed that the putative transporter genes open reading frame (ORF) 102-105 were upregulated in cells grown on the monoterpen phellandren. The proteins were assigned to the RND-transport system which can decrease the intracellular concentration of toxic compounds to avoid cell damage. To determine the function of the transporter the deletion mutant Castellaniella defragrans ΔORF 102-105 was created. Growth experiments showed that the transporter genes import the monoterpen R-(+)-limonene. There was no difference in transport of monoterpenoides from the wild type compared to the deletion mutant.