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Journal Article

Crystal ball: Fluorescence in situ hybridization in the age of super-resolution microscopy

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Moraru,  C.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Amann,  R.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Citation

Moraru, C., & Amann, R. (2012). Crystal ball: Fluorescence in situ hybridization in the age of super-resolution microscopy. Systematic and Applied Microbiology, 35(8), 549-552.


Cite as: https://hdl.handle.net/21.11116/0000-0001-C786-7
Abstract
Super-resolution microscopy encompasses a suite of cutting edge microscopy methods able to surpass the resolution limits of light microscopy. The recent commercial availability of super-resolution microscopy is advancing many fields of biology. In this crystal ball forward look, we briefly examine the perspectives of combining super-resolution microscopy and fluorescence in situ hybridization (FISH). We strongly believe, based on first evidence presented here, that using super-resolution microscopy in environmental microbiology has the potential to reshape the way we analyze the results obtained with FISH, by improving both the localization and quantification of target molecules.

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