Lectin binding in Cryptomonas and Chroomonas (Cryptophyceae)

Rhiel, E.; Brock, J.

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Lectin binding in Cryptomonas and Chroomonas (Cryptophyceae)

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Brock, J.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Citation

Rhiel, E., & Brock, J. (2012). Lectin binding in Cryptomonas and Chroomonas (Cryptophyceae). Protoplasma, 249(3), 759-768.

Cite as: https://hdl.handle.net/21.11116/0000-0001-C7FF-0

Abstract

The cell envelopes of Cryptomonas and Chroomonas exhibited significant fluorescence using FITC-labelled concanavalin A and wheat germ agglutinin when the cells were fixed prior to lectin binding. The periplast became intensely labelled in Chroomonas whereas Cryptomonas showed fluorescing granula in its gullet/furrow region and on the cell surface. Lectin labelling followed by fixation showed only label of periplast remnants of lysed cells and of the flagella of Chroomonas. Isolated periplasts of Cryptomonas and Chroomonas were intensively labelled with both concanavalin A and wheat germ agglutinin. Glycostaining of gels, onto which total cell protein extracts were loaded, showed a glycoprotein of high molecular weight for Cryptomonas and Chroomonas and an additional glycoprotein for Cryptomonas species.