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Nitrogen fixation and transfer in open ocean diatom-cyanobacterial symbioses

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Foster,  R. A.
Department of Biogeochemistry, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Kuypers,  M. M. M.
Department of Biogeochemistry, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Vagner,  T.
Department of Biogeochemistry, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Musat,  N.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Citation

Foster, R. A., Kuypers, M. M. M., Vagner, T., Paerl, R. W., Musat, N., & Zehr, J. P. (2011). Nitrogen fixation and transfer in open ocean diatom-cyanobacterial symbioses. The ISME Journal, 5(9), 1484-1493.


Cite as: http://hdl.handle.net/21.11116/0000-0001-C91F-B
Abstract
Many diatoms that inhabit low-nutrient waters of the open ocean live in close association with cyanobacteria. Some of these associations are believed to be mutualistic, where N2-fixing cyanobacterial symbionts provide N for the diatoms. Rates of N2 fixation by symbiotic cyanobacteria and the N transfer to their diatom partners were measured using a high-resolution nanometer scale secondary ion mass spectrometry approach in natural populations. Cell-specific rates of N2 fixation (1.15–71.5 fmol N per cell h−1) were similar amongst the symbioses and rapid transfer (within 30 min) of fixed N was also measured. Similar growth rates for the diatoms and their symbionts were determined and the symbiotic growth rates were higher than those estimated for free-living cells. The N2 fixation rates estimated for Richelia and Calothrix symbionts were 171–420 times higher when the cells were symbiotic compared with the rates estimated for the cells living freely. When combined, the latter two results suggest that the diatom partners influence the growth and metabolism of their cyanobacterial symbionts. We estimated that Richelia fix 81–744% more N than needed for their own growth and up to 97.3% of the fixed N is transferred to the diatom partners. This study provides new information on the mechanisms controlling N input into the open ocean by symbiotic microorganisms, which are widespread and important for oceanic primary production. Further, this is the first demonstration of N transfer from an N2 fixer to a unicellular partner. These symbioses are important models for molecular regulation and nutrient exchange in symbiotic systems.