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Journal Article

Highly enriched Betaproteobacteria growing anaerobically with p-xylene and nitrate

MPS-Authors
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Rotaru,  A. E.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Probian,  C.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Wilkes,  H.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Harder,  J.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Rotaru10.pdf
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Citation

Rotaru, A. E., Probian, C., Wilkes, H., & Harder, J. (2010). Highly enriched Betaproteobacteria growing anaerobically with p-xylene and nitrate. FEMS Microbiology Ecology, 71(3), 460-468.


Cite as: http://hdl.handle.net/21.11116/0000-0001-CAFA-2
Abstract
The identity of the microorganisms capable of anaerobic p-xylene degradation under denitrifying conditions is hitherto unknown. Here, we report highly enriched cultures of freshwater denitrifying bacteria that grow anaerobically with p-xylene as the sole organic carbon source and electron donor. Long curved rods, with 95% 16S rRNA gene sequence identity to Denitratisoma oestradiolicum, dominated the enrichment cultures (>91% of all cells), as detected by phylotype-specific probes. These Rhodocyclaceae microorganisms were distantly related to other denitrifying hydrocarbon-degrading Betaproteobacteria from the Azoarcus-Thauera clade. Complete oxidation p-xylene to CO(2) coupled to denitrification was suggested by quantitative measurements of substrate consumption. Metabolite analysis identified (4-methylbenzyl)succinate and (4-methylphenyl)itaconate, suggesting addition to fumarate as an initial activation reaction.