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Identification of the dominant sulfate-reducing bacterial partner of anaerobic methanotrophs of the ANME-2 clade

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Schreiber,  Lars
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Holler,  Thomas
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Knittel,  Kartin
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Meyerdierks,  Anke
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Amann,  Rudolf
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Schreiber10.pdf
(Publisher version), 519KB

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Citation

Schreiber, L., Holler, T., Knittel, K., Meyerdierks, A., & Amann, R. (2010). Identification of the dominant sulfate-reducing bacterial partner of anaerobic methanotrophs of the ANME-2 clade. Environmental Microbiology, 12(8), 2327-2340.


Cite as: http://hdl.handle.net/21.11116/0000-0001-CB82-7
Abstract
The anaerobic oxidation of methane (AOM) with sulfate as terminal electron acceptor is mediated by consortia of methanotrophic archaea (ANME) and sulfate-reducing bacteria (SRB). Whereas three clades of ANME have been repeatedly studied with respect to phylogeny, key genes and genomic capabilities, little is known about their sulfate-reducing partner. In order to identify the partner of anaerobic methanotrophs of the ANME-2 clade, bacterial 16S rRNA gene libraries were constructed from cultures highly enriched for ANME-2a and ANME-2c in consortia with Deltaproteobacteria of the Desulfosarcina/Desulfococcus group (DSS). Phylogenetic analysis of those and publicly available sequences from AOM sites supported the hypothesis by Knittel and colleagues that the DSS partner belongs to the diverse SEEP-SRB1 cluster. Six subclusters of SEEP-SRB1, SEEP-SRB1a to SEEP-SRB1f, were proposed and specific oligonucleotide probes were designed. Using fluorescence in situ hybridization on samples from six different AOM sites, SEEP-SRB1a was identified as sulfate-reducing partner in up to 95% of total ANME-2 consortia. SEEP-SRB1a cells exhibited a rod-shaped, vibrioid, or coccoid morphology and were found to be associated with subgroups ANME-2a and ANME-2c. Moreover, SEEP-SRB1a was also detected in 8% to 23% of ANME-3 consortia in Haakon Mosby Mud Volcano sediments, previously described to be predominantly associated with SRB of the Desulfobulbus group. SEEP-SRB1a contributed to only 0.3% to 0.7% of all single cells in almost all samples indicating that these bacteria are highly adapted to a symbiotic relationship with ANME-2.