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Solvent stress response of the denitrifying bacterium "Aromatoleum aromaticum" strain EbN1

MPS-Authors
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Trautwein,  K.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Kuehner,  S.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Woehlbrand,  L.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Rabus,  R.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Trautwein8.pdf
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Citation

Trautwein, K., Kuehner, S., Woehlbrand, L., Halder, T., Kuchta, K., Steinbucehel, A., et al. (2008). Solvent stress response of the denitrifying bacterium "Aromatoleum aromaticum" strain EbN1. Applied and Environmental Microbiology, 74(8), 2267-2274.


Cite as: http://hdl.handle.net/21.11116/0000-0001-CD9A-B
Abstract
The denitrifying betaproteobacterium “Aromatoleum aromaticum” strain EbN1 degrades several aromatic compounds, including ethylbenzene, toluene, p-cresol, and phenol, under anoxic conditions. The hydrophobicity of these aromatic solvents determines their toxic properties. Here, we investigated the response of strain EbN1 to aromatic substrates at semi-inhibitory (about 50% growth inhibition) concentrations under two different conditions: first, during anaerobic growth with ethylbenzene (0.32 mM) or toluene (0.74 mM); and second, when anaerobic succinate-utilizing cultures were shocked with ethylbenzene (0.5 mM), toluene (1.2 mM), p-cresol (3.0 mM), and phenol (6.5 mM) as single stressors or as a mixture (total solvent concentration, 2.7 mM). Under all tested conditions impaired growth was paralleled by decelerated nitrate-nitrite consumption. Additionally, alkylbenzene-utilizing cultures accumulated poly(3-hydroxybutyrate) (PHB) up to 10% of the cell dry weight. These physiological responses were also reflected on the proteomic level (as determined by two-dimensional difference gel electrophoresis), e.g., up-regulation of PHB granule-associated phasins, cytochrome cd1 nitrite reductase of denitrification, and several proteins involved in oxidative (e.g., SodB) and general (e.g., ClpB) stress responses.