Evidence for chemoautotrophic symbiosis in a Mediterranean cold seep clam 
(Bivalvia : Lucinidae): comparative sequence analysis of bacterial 16S rRNA, 
APS reductase and RubisCO genes

Duperron, S.; Fiala-Medioni, A.; Caprais, J. C.; Olu, K.; Sibuet, M.

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Evidence for chemoautotrophic symbiosis in a Mediterranean cold seep clam (Bivalvia : Lucinidae): comparative sequence analysis of bacterial 16S rRNA, APS reductase and RubisCO genes

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Duperron, S.
Microbial Habitat Group, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Duperron, S., Fiala-Medioni, A., Caprais, J. C., Olu, K., & Sibuet, M. (2007). Evidence for chemoautotrophic symbiosis in a Mediterranean cold seep clam (Bivalvia: Lucinidae): comparative sequence analysis of bacterial 16S rRNA, APS reductase and RubisCO genes. FEMS Microbiology Ecology, 59(1), 64-70.

Cite as: https://hdl.handle.net/21.11116/0000-0001-CEA8-A

Abstract

Symbioses between lucinid clams (Bivalvia: Lucinidae) and autotrophic sulphide-oxidizing bacteria have mainly been studied in shallow coastal species, and information regarding deep-sea species is scarce. Here we study the symbiosis of a clam, resembling Lucinoma kazani, which was recently collected in sediment cores from new cold-seep sites in the vicinity of the Nile deep-sea fan, eastern Mediterranean, at depths ranging from 507 to 1691 m. A dominant bacterial phylotype, related to the sulphide-oxidizing symbiont of Lucinoma aequizonata, was identified in gill tissue by comparative 16S rRNA gene sequence analysis. A second phylotype, related to spirochete sequences, was identified twice in a library of 94 clones. Comparative analyses of gene sequences encoding the APS reductase alpha subunit and ribulose-1,5-bisphosphate carboxylase oxygenase support the hypothesis that the dominant symbiont can perform sulphide oxidation and autotrophy. Transmission electron micrographs of gills confirmed the dominance of sulphide-oxidizing bacteria, which display typical vacuoles, and delta(13)C values measured in gill and foot tissue further support the hypothesis for a chemoautotrophic-sourced host carbon nutrition.