A CARD-FISH protocol for the identification and enumeration of epiphytic 
bacteria on marine algae

Tujula, N. A.; Holmstrom, C.; Mussmann, M.; Amann, R.; Kjelleberg, S.; Crocetti, G. R.

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A CARD-FISH protocol for the identification and enumeration of epiphytic bacteria on marine algae

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Mussmann, M.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Amann, R.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Citation

Tujula, N. A., Holmstrom, C., Mussmann, M., Amann, R., Kjelleberg, S., & Crocetti, G. R. (2006). A CARD-FISH protocol for the identification and enumeration of epiphytic bacteria on marine algae. Journal of Microbiological Methods, 65(3), 604-607.

Cite as: https://hdl.handle.net/21.11116/0000-0001-CF5F-D

Abstract

A CARD-FISH protocol was developed and applied to analyse surface-associated bacteria on the marine algae Ulva lactuca, Delisea pulchra, Corallina officinalis, Amphiroa anceps, Porphyra sp. and Sargassum linearifolium. The combination of Alexa(546)-labelled tyramide as the reporter molecule with SYBR Green II counterstain allowed for superior detection of the hybridised probe fluorescence against plant tissue from which pigment autofluorescence has been reduced.