English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

The effect of nucleobase-specific fluorescence quenching on in situ hybridization with rRNA-targeted oligonucleotide probes

MPS-Authors
/persons/resource/persons210261

Behrens,  S.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

/persons/resource/persons210389

Fuchs,  B. M.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

/persons/resource/persons210230

Amann,  R.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

External Ressource
No external resources are shared
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Behrens, S., Fuchs, B. M., & Amann, R. (2004). The effect of nucleobase-specific fluorescence quenching on in situ hybridization with rRNA-targeted oligonucleotide probes. Systematic and Applied Microbiology, 27(5), 565-572.


Cite as: http://hdl.handle.net/21.11116/0000-0001-D10D-5
Abstract
Oligonucleotide probes labeled with fluorescent dyes are used in a variety of in situ applications to detect specific DNA or RNA molecules. It has been described that probe fluorescence might be quenched upon hybridization in a sequence specific way. Here, a set of 17 oligonuleotides labeled with 6-carboxyfluorescein was used to examine the relevance of nucleotide specific quenching for fluorescence in situ hybridization (FISH) to whole fixed bacterial cells. Probes quenched upon hybridization to a guanine-rich region of purified RNA in solution were not quenched upon FISH. Among other factors the high protein concentration within cells may prevent quenching of probe fluorescence in situ.