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Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum gryphiswaldense reveals a large deletion comprising a putative magnetosome island

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Schübbe,  S.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Kube,  M.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Scheffel,  A.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Wawer,  C.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Heyen,  U.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Meyerdierks,  A.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Reinhardt,  R.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Schüler,  D.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Citation

Schübbe, S., Kube, M., Scheffel, A., Wawer, C., Heyen, U., Meyerdierks, A., et al. (2003). Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum gryphiswaldense reveals a large deletion comprising a putative magnetosome island. Journal of Bacteriology, 185(19), 5779-5790.


Cite as: https://hdl.handle.net/21.11116/0000-0001-D1EF-6
Abstract
Frequent spontaneous loss of the magnetic phenotype was observed in stationary-phase cultures of the magnetotactic bacterium Magnetospirillum gryphiswaldense MSR-1. A nonmagnetic mutant, designated strain MSR-1B, was isolated and characterized. The mutant lacked any structures resembling magnetosome crystals as well as internal membrane vesicles. The growth of strain MSR-1B was impaired under all growth conditions tested, and the uptake and accumulation of iron were drastically reduced under iron-replete conditions. A large chromosomal deletion of approximately 80 kb was identified in strain MSR-1B, which comprised both the entire mamAB and mamDC clusters as well as further putative operons encoding a number of magnetosome-associated proteins. A bacterial artificial chromosome clone partially covering the deleted region was isolated from the genomic library of wild-type M. gryphiswaldense. Sequence analysis of this fragment revealed that all previously identified mam genes were closely linked with genes encoding other magnetosome-associated proteins within less than 35 kb. In addition, this region was remarkably rich in insertion elements and harbored a considerable number of unknown gene families which appeared to be specific for magnetotactic bacteria. Overall, these findings suggest the existence of a putative large magnetosome island in M. gryphiswaldense and other magnetotactic bacteria.