Molecular quantification of genes encoding for green-fluorescent proteins

Felske, A.; Vandieken, V.; Pauling, B. V.; von Canstein, H. F.; Wagner-Dobler, I.

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Molecular quantification of genes encoding for green-fluorescent proteins

MPS-Authors

/persons/resource/persons210830

Vandieken, V.
Department of Biogeochemistry, Max Planck Institute for Marine Microbiology, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Felske, A., Vandieken, V., Pauling, B. V., von Canstein, H. F., & Wagner-Dobler, I. (2003). Molecular quantification of genes encoding for green-fluorescent proteins. Journal of Microbiological Methods, 52(3), 297-304.

Cite as: https://hdl.handle.net/21.11116/0000-0001-D249-0

Abstract

A quantitative PCR approach is presented to analyze the amount of recombinant green fluorescent protein (gfp) genes in environmental DNA samples. The quantification assay is a combination of specific PCR amplification and temperature gradient gel electrophoresis (TGGE). Gene quantification is provided by a competitively coamplified DNA standard constructed by point mutation PCR. A single base difference was introduced to achieve a suitable migration difference in TGGE between the original target DNA and the modified standard without altering the PCR amplification efficiency. This competitive PCR strategy is a highly specific and sensitive way to monitor recombinant DNA in environments like the efflux of a biotechnological plant.