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Journal Article

Structural changes in sarcoplasmic reticulum membrane induced by SH reagents


Hasselbach,  Wilhelm
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Dupont, Y., & Hasselbach, W. (1973). Structural changes in sarcoplasmic reticulum membrane induced by SH reagents. Nature New Biology, 246, 41-44. doi:10.1038/newbio246041a0.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002E-0513-4
Using sarcoplasmic reticulum (SR) membranes, stacked and orientated by centrifugation, Dupont, Harrison and Hasselbach1 demonstrated that it was possible to record Bragg reflections to 15 Å−1 resolution. The phases of the reflections and the electron density profiles were obtained to a resolution of 30 Å. In an attempt to label the calcium pump protein with heavy atoms very dramatic changes were observed in the X-ray diffraction pattern when organo-mereurials or other SH reagents were bound to the ATPase. These changes seem to have been due to large structural modifications of the membrane.