English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Book Chapter

Analysis of Yeast Mitochondria by Electron Microscopy

MPS-Authors
/persons/resource/persons78450

Neupert,  Walter
Neupert, Walter / Structure and Function of Mitochondria, Max Planck Institute of Biochemistry, Max Planck Society;

Locator
There are no locators available
Fulltext (public)
There are no public fulltexts available
Supplementary Material (public)
There is no public supplementary material available
Citation

Unger, A.-K., Geimer, S., Harner, M., Neupert, W., & Westermann, B. (2017). Analysis of Yeast Mitochondria by Electron Microscopy. In D. Mokranjac, & F. Perocchi (Eds.), Mitochondria (2nd ed., pp. 293-314). New York, NY: Humana Press.


Cite as: http://hdl.handle.net/21.11116/0000-0000-7807-2
Abstract
Budding yeast Saccharomyces cerevisiae represents a widely used model organism for the study of mitochondrial biogenesis and architecture. Electron microscopy is an essential tool in the analysis of cellular ultrastructure and the precise localization of proteins to organellar subcompartments. We provide here detailed protocols for the analysis of yeast mitochondria by transmission electron microscopy: (1) chemical fixation and Epon embedding of yeast cells and isolated mitochondria, and (2) cryosectioning and immunolabeling of yeast cells and isolated mitochondria according to the Tokuyasu method.