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Journal Article

The action of chaotropic anions on the sarcoplasmic calcium pump

MPS-Authors
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The,  Rudy
Max Planck Institute for Medical Research, Max Planck Society;

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Hasselbach,  Wilhelm
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1975.tb04047.x/epdf
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https://doi.org/10.1111/j.1432-1033.1975.tb04047.x
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Citation

The, R., & Hasselbach, W. (1975). The action of chaotropic anions on the sarcoplasmic calcium pump. European Journal of Biochemistry, 53(1), 105-113. doi:10.1111/j.1432-1033.1975.tb04047.x.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002E-0B86-0
Abstract
1The effect of the chaotropic agents on three different preparations of the sarcoplasmic reticulum, i.e. native membranes, membranes whose phospholipids were hydrolyzed by phospholipase A and lipid-deficient membranes has been studied.
2The chaotropic anions inhibit calcium transport, calcium-dependent ATPase activity, phosphoprotein formation and ATP binding.The effectiveness of the anions increases in the following order:
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3Inhibition occurs immediately and is reversible up to an incubation period of 7 min.
4The maximal effective concentration of the chaotropic anions (0.4 M) does not solubilize either membrane protein or membrane lipids.
5The chaotropic anions increase the calcium-permeability of the sarcoplasmic membranes a relatively small amount, so that the permeability increase cannot account for the reduced calcium uptake.
6The interference between the binding of ATP and chaotropic anions is the most likely mechanism of their action.