Deutsch
 
Benutzerhandbuch Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Protoplast swelling and hypocotyl growth depend on different auxin signaling pathways

MPG-Autoren
/persons/resource/persons201528

Ullrich,  Kristian K.
Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society;

Externe Ressourcen

Link
(beliebiger Volltext)

Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Dahlke, R. I., Fraas, S., Ullrich, K. K., Heinemann, K., Romeiks, M., Rickmeyer, T., et al. (2017). Protoplast swelling and hypocotyl growth depend on different auxin signaling pathways. Plant Physiology, 175(2), 982-994. doi:10.1104/pp.17.00733.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-002E-0E11-D
Zusammenfassung
Members of the TRANSPORT INHIBITOR RESPONSE1/AUXIN SIGNALING F-BOX PROTEIN (TIR1/AFB) family are known auxin receptors. To analyze the possible receptor function of AUXIN BINDING PROTEIN1 (ABP1), an auxin receptor currently under debate, we performed different approaches. We performed a pharmacological approach using α-(2,4-dimethylphenylethyl-2-oxo)-indole-3-acetic acid (auxinole), α-(phenylethyl-2-oxo)-indole-3-acetic acid (PEO-IAA), and 5-fluoroindole-3-acetic acid (5-F-IAA) to discriminate between ABP1- and TIR1/AFB-mediated processes in Arabidopsis (Arabidopsis thaliana). We used a peptide of the carboxyl-terminal region of AtABP1 as a tool. We performed mutant analysis with the null alleles of ABP1, abp1-c1 and abp1-TD1, and the TILLING mutant abp1-5. We employed Coimbra, an accession that exhibits an amino acid exchange in the auxin-binding domain of ABP1. We measured either volume changes of single hypocotyl protoplasts or hypocotyl growth, both at high temporal resolution. 5-F-IAA selectively activated the TIR1/AFB pathway but did not induce protoplast swelling; instead, it showed auxin activity in the hypocotyl growth test. In contrast, PEO-IAA induced an auxin-like swelling response but no hypocotyl growth. The carboxyl-terminal peptide of AtABP1 induced an auxin-like swelling response. In the ABP1-related mutants and Coimbra, no auxin-induced protoplast swelling occurred. ABP1 seems to be involved in mediating rapid auxin-induced protoplast swelling, but it is not involved in the control of rapid auxin-induced growth.GlossaryFCfusicoccin1-NAA1-naphthaleneacetic acidMOEMolecular Operating Environment2-NAA2-naphthaleneacetic acidERendoplasmic reticulumCol-0Columbia-0Co-1CoimbraSNPsingle-nucleotide polymorphism