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Adaptive evolution by spontaneous domain fusion and protein re-localization

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Remigi,  Philippe
Department Microbial Population Biology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Rainey,  Paul B.       
Department Microbial Population Biology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Citation

Farr, A. D., Remigi, P., & Rainey, P. B. (2017). Adaptive evolution by spontaneous domain fusion and protein re-localization. Nature Ecology & Evolution, 1, 1562-1568. doi:10.1038/s41559-017-0283-7.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002E-263C-2
Abstract
Knowledge of adaptive processes encompasses understanding the emergence of new genes. Computational analyses of genomes suggest that new genes can arise by domain swapping; however, empirical evidence has been lacking. Here we describe a set of nine independent deletion mutations that arose during selection experiments with the bacterium Pseudomonas fluorescens in which the membrane-spanning domain of a fatty acid desaturase became translationally fused to a cytosolic di-guanylate cyclase, generating an adaptive 'wrinkly spreader' phenotype. Detailed genetic analysis of one gene fusion shows that the mutant phenotype is caused by relocalization of the di-guanylate cyclase domain to the cell membrane. The relative ease by which this new gene arose, along with its functional and regulatory effects, provides a glimpse of mutational events and their consequences that are likely to have a role in the evolution of new genes. © 2017 The Author(s).